Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain -- Data Supplement - JCB--Nezis et al. -- The Journal of Cell Biology

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Figure S1. Accumulation of protein deposits in the brains of old flies, proteasome mutant flies, and tau-expressing flies. (A and B) Confocal micrographs of fixed adult brain of old (8 wk old) wild-type fly without staining. Very few autofluorescent deposits can be observed. (C) Western blot analysis of insoluble protein fractions of adult heads of proteasome mutants probed for ref(2)P, demonstrating a significant increase in the levels of Ref(2)P protein at the restrictive temperature of 29°C. (D) Western blot analysis of insoluble protein fractions of adult heads of tau mutants probed for tau and Ref(2)P, demonstrating tau expression and a significant increase in the levels of Ref(2)P protein in the tau-expressing flies. Bars, 100 µm.