[View Larger Version of this Image]

Figure S2. 12-HHT–induced intracellular signaling is mediated by BLT2. (A) IP accumulation assay using COS-7 cells cotransfected with BLT2 and various subunits of Gq proteins. (B) IP assay using COS-7 cells cotransfected with human BLT2 (hBLT2) or mouse BLT2 (mBLT2) and G14 (mean ± SEM; n = 3). (C and D) The effects of 100 ng/ml PTX pretreatment on 12-HHT–dependent adenylate cyclase inhibition (C; mean ± SEM; n = 4) and chemotaxis (D; mean ± SEM; n = 3) through BLT2.