[View Larger Version of this Image]
Figure S1. Chemotaxis mediated by chemerin, chemerin peptides, and chemerin-treated supernatants. (A–G) PMΦs (0.4 × 106) with or without 30-min pertussis toxin pretreatment (PTX; 200 ng/ml) were allowed to migrate toward chemoattractant (A, rmChemerin; B, C15; C, C11; D, C13; E, C19; F, C6; G, C8) in the bottom well of a modified Boyden chamber over 4 h. (H) PMΦs (7.5 × 105) were allowed to migrate toward conditioned media from untreated macrophages and macrophages treated with LPS/IFNγ ± chemerin or C15 in the bottom well of a modified Boyden chamber over 4 h. Graphs indicate mean migration index ± SEM for each treatment group (n = 4 independent experiments). Checkerboard analysis showed that chemerin and chemerin-derived peptides induce true chemotaxis rather than chemokinesis. ***, P < 0.001; **, P < 0.01; *, P < 0.05 relative to PMΦs + PTX.