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Figure S3. Chemerin15 suppresses monocyte and neutrophil recruitment in zymosan peritonitis over a range of doses. Mice (five to six animals/treatment) were dosed i.p. with PBS or C15 dose range (4–40 pg/mouse), followed by injection with PBS or zymosan (10 µg) 1 h later. Peritoneal exudate cells were harvested by peritoneal lavage 4 h after zymosan challenge (five to six mice/group). Total cell numbers in lavage fluid were quantified, and cellular composition (neutrophils vs. mononuclear phagocytes) was determined using FACS analysis as described in Fig. 3. C15, chemerin15; Z, zymosan. ***, P < 0.001; **, P < 0.01 **; *, P < 0.05 relative to zero pg/mouse C15, one-way ANOVA with Tukeys post test).