Blood, Vol. 111, Issue 9, 4797-4808, May 1, 2008

Somatic mutations and germline sequence variants in the expressed tyrosine kinase genes of patients with de novo acute myeloid leukemia
Blood Tomasson et al. 111: 4797

Supplemental materials for: Tomasson et al

Files in this Data Supplement:

• Table S1. Re-sequencing primers used for RTK and CTK genes (PDF, 109 KB)
  
  
• Table S2. Oligonucleotide primers used for pyrosequencing (PDF, 10.7 KB)
  
  
• Table S3. Prioritization of receptor tyrosine kinase (RTK) (A) and cytoplasmic tyrosine kinase (CTK) (B) genes by expression in AML cases (PDF, 71.1 KB)
  
  
• Table S4. Re-sequencing coverage of RTK and CTK genes (PDF, 17.6 KB)
  
  
• Figure S1. Sequence traces for NTRK1^S677N and DDR1^A803V somatic mutations (JPG, 108 KB) -
  Both sequence variants are seen reproducibly in forward and reverse reads from the bone marrow samples (tumor), but not in traces from skin DNA reactions (germline).
  
  
  
  
  
  
• Figure S2. Non-synonymous base changes identified by exonic re-sequencing of DNAs from 94 AML patients in validation (CALGB) set (JPG, 386 KB) -
  Patient Numbers are shown in the rows, and the names of sequenced genes are shown in the columns. Green color indicates that no non-synonymous sequence variants were identified in that sample. Red color identifies samples with non-synonymous variants; the predicted consequences of all sequence variants detected are listed. Yellow color indicates the presence of a homozygous sequence variant. White boxes indicate that no sequence was obtained. All somatic mutations were confirmed by automated resequencing and/or hand validation.