Video 7
Kinetochore MT bundles are defective after RNAi of double Cnn/Dgt5. Cells expressing GFP-tubulin and Mis12-mCherry were treated with double-stranded RNAs of Cnn and Dgt5 for 4 d, and images were acquired every 30 s immediately after colcemid addition. A wide-field fluorescence microscope with a 100× objective lens was used.