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Figure S3. Chromosome misalignment and kMT defects after Dgt RNAi. (A) Frequency of bipolar spindles that have unaligned chromosomes. Error bars represent SE of two experiments. Moderate increase was observed after Dgt RNAi. Control, 30% (n = 230); Dgt2, 55% (n = 67); Dgt3, 42% (n = 150); Dgt4, 46% (n = 115); Dgt5, 41% (n = 128); Dgt6, 47% (n = 148); γ-tubulin, 76% (n = 64); Dgrip71, 46% (n = 86); and Cal1, 79% (n = 86). (B, left) A still image of GFP-tubulin (green) and Mis12-mCherry (red) after single Cnn and double Cnn/Dgt5 RNAi. (B, right) 10 min after colcemid addition, kMT bundles (K-fibers) were disorganized (see also Video 7). Bars, 5 µm.