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Figure S2. Impaired generation of memory Th1 cells from Bmi1^−/− effector Th1 cells in BALB/c mice. Bmi1^+/+, Bmi1^+/−, and Bmi1^−/− effector Th2 cells with a DO11. 10 Tg background were intravenously transferred into BALB/c mice (n = 5). 5 wk later, the number of KJ1⁺ memory Th2 cells was determined by flow cytometry. Typical staining patterns of KJ1/CD4 and the percentages of KJ1⁺CD4⁺ cells are shown. Three independent experiments were performed with similar results.