T cell receptor-instructed {alpha}{beta} versus {gamma}{delta} lineage commitment revealed by single-cell analysis -- Data Supplement - JEM--Kreslavsky et al. -- The Journal of Experimental Medicine

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Figure S1. DN2 cells do not express surface TCRγδ. Thymocytes from pTα^−/− and WT mice were stained for CD4, CD8, CD25, TCRγδ, and cKit and analyzed by means of flow cytometry. Plots show TCRγδ and cKit expression in DN2/DN3 cells (CD4⁻CD8⁻CD25⁺).