Index of Online Supplemental Material for J. Exp. Med. 10.1084/jem.20072168Benz et al. Figure S1 The scheme illustrates the two-step transplantation strategy.
Figure S2 Lin− CD45.2low/positive T lineage precursors isolated from transplanted thymic lobes were sorted as single cells or groups of cells onto OP9-DL1 stromal layers.
Figure S3 Generation of Flt3::Cre transgenic mice.
Figure S4 An inducible lineage-tracing approach shows that the stream of precursors proceeds from the bone marrow selectively through the thymic ETP population.
Figure S5 FACS analysis of CCR9EGFP+ LSKs in bone marrow (BM) and peripheral blood (PB) of CCR9-EGFP knock-in mice for Flt3 expression.
Table S1 (PDF)