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Figure S2. The typical frequency decreases for each PABP-binding molecule (left), and the linear reciprocal plots of the relaxation time τ against the concentration of PABP (right). The frequency decreases based on Equation 3 indicate the time dependence of the increase in mass due to bound molecules (left). The k_on and k_off values were obtained using Equation 4 (see Materials and methods). The K_d values of Msi1 or eIF4G to PABP were estimated from the ratio of k_off to k_on. The significance of this affinity difference is addressed in the Materials and methods.