Carrasco et al. 10.1073/pnas.0708017105. |
Fig. 6. Electron micrographs of purified empty-capsid preparations. (Left) Nonmutated (WT). (Center) D58A mutant. (Right) N183A mutant.
Fig. 7. Electron micrographs of purified virion preparations. (Left) Nonmutated (WT). (Center) D58A mutant. (Right) N183A mutant.
Fig. 8. Averaged indentation curves and typical deflection curves for nonmutated (a), N183A (b), and D58A (c) empty capsids probed along an S2, S3, or S5 axis. Each indentation graph is the average of at least five curves. The deflection curves shown were obtained during one of these measurements. The thin line corresponds to a typical deflection curve when the glass support was probed.
Fig. 9. Averaged indentation curves and typical deflection curves for nonmutated (a), N183A (b), and D58A (c) virions probed along an S2, S3, or S5 axis. The curves were obtained as described in the Fig. 8 legend.
Table 1. Spring constants k obtained in independent experiments ["Old" (1) and "New"], using different preparations of nonmutated MVM particles
Symmetry | Empty capsid | Virion | ||
Old k, N/m | New k,N/m | Old k, N/m | New k, N/m | |
S5 | 0.58 ± 0.13 | 0.57 ± 0.06 | 0.6 ± 0.2 | 0.53 ± 0.12 |
S3 | 0.56 ± 0.15 | 0.54 ± 0.15 | 0.8 ± 0.4 | 0.84 ± 0.12 |
S2 | 0.58 ± 0.10 | 0.56 ± 0.05 | 1.4 ± 0.5 | 1.19 ± 0.34 |
1. Carrasco C, Carreira A, Schaap IAT, Serena PA, Gómez-Herrero J, Mateu MG, de Pablo PJ (2006) Proc Natl Acad Sci USA 103:13706-13711.
Table 2. Number of viral particles probed and (in parentheses) total number of indentations along the different types of symmetry axes (S5, S3, S2)
Symmetry | Capsids | Virions | ||||
Nonmutated | 183A | 58A | Nonmutated | 183A | 58A | |
S5 | 9 (53) | 5 (44) | 5 (44) | 14 (100) | 8 (34) | 6 (64) |
S3 | 17 (100) | 7 (59) | 12 (100) | 22 (110) | 15 (79) | 7 (66) |
S2 | 7 (34) | 6 (63) | 5 (42) | 11 (90) | 12 (88) | 5 (46) |