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Catalytic constants with all-trans-4-OH-retinaldehyde in Tween 80
|
kcat, min−1 |
||
|---|---|---|
| all-trans-Retinaldehyde | (S)-all-trans-4-Hydroxyretinaldehyde | |
| AKR1B1 | 0.37 ± 0.02 | 5.80 ± 0.16 |
| AKR1B10 | 17.8 ± 1.0 | 17.3 ± 1.5 |
| AKR1B10 K125L | 4.40 ± 0.02 | 18.5 ± 2.6 |
Activities were performed in 0.1 M sodium phosphate (pH 7.5)/0.2 mM NADPH/0.02% Tween 80 at 25°C (7).
