[View Larger Version of this Image]
Figure S4. Absence of production of IL-12 p75 and p40 in mono-DCs from three NOD2−/− Crohn’s disease patients in response to H37Rv. Mono-DCs were labeled with [35S]methionine and stimulated with heat-killed H37Rv, MDP, R848, MDP+R848, or LPS+R848. After 18 h, supernatants were immunoprecipitated with anti–IL-12 p35 or anti–IL-12/23 p40 mAbs and resolved in nonreducing SDS-PAGE. Crohn’s disease patients and control donors of mono-DCs examined for IL-12 p75 correspond, respectively, to the same donors of samples analyzed for p40.