Supplementary material for Adams et al. (1999) Proc. Natl. Acad. Sci. USA 96 (24), 13863-13868.
Table 2.
PCR and RT-PCR primersSequence (5' to 3') | Orientation/priming site |
1. CARGGGCCTGCTATCTCRTKTTAGG | Forward/nuclear cox2 targeting sequence |
2. GGAARCWYTGGCATRTCWAGATT | Forward/nuclear cox2 targeting sequence |
3. GKTGATTTYATCYGGTGSTA | Forward/nuclear cox2 targeting sequence |
4. CAASTTGATTTCATCTDGTGG | Forward/nuclear cox2 targeting sequence |
5: TGAACCCTGAACARRTTGTTC | Forward/nuclear cox2 targeting sequence |
6: CCAYCTCAGTAGTTATATTAT | Reverse/nuclear cox2 targeting sequence |
7. CTTGAGACCTCTGGTGTCTT | Reverse/nuclear cox2 targeting sequence |
8. TACCATTGATGYCCAATAGC | Reverse/mature coding region of nuclear cox2 |
9. CCAATACCATTGATGYCCAA | Reverse/mature coding region of nuclear cox2 |
10. MCCAGGTACWGCATCACNTT | Reverse/mature coding region of nuclear cox2 |
11. ACAGCTTCYACAACRATAGG | Reverse/mature coding region of nuclear cox2 |
12. AWACCCADGAASCATAATC | Reverse/mature coding region of nuclear cox2 |
13. GGGATTAKTKGAHTTGAYTG | Reverse/mature coding region of nuclear cox2 |
14. CCATACTACAAAACCCCCAG | Reverse/mature coding region of Amphicarpa nuclear cox2 |
15. GAGCTATCGTAGGCATCAAT | Reverse/mature coding region of Pachyrhizus nuclear cox2 |
16. CCCAACCAGCGATTTACAACAA | Forward/5' UTR of mitochondrial cox2 |
17. TTGTTGGGGGAAGCAGTTA | Forward/5' UTR of mitochondrial cox2 |
18. CCTACTCTTTCATTTCCGC | Reverse/3' UTR of mitochondrial cox2 |
UTR, untranslated region.