Supplementary material for Adams et al. (1999) Proc. Natl. Acad. Sci. USA 96 (24), 13863-13868.

Table 2.

PCR and RT-PCR primers

Sequence (5' to 3')

Orientation/priming site

1. CARGGGCCTGCTATCTCRTKTTAGG

Forward/nuclear cox2 targeting sequence

2. GGAARCWYTGGCATRTCWAGATT

Forward/nuclear cox2 targeting sequence

3. GKTGATTTYATCYGGTGSTA

Forward/nuclear cox2 targeting sequence

4. CAASTTGATTTCATCTDGTGG

Forward/nuclear cox2 targeting sequence

5: TGAACCCTGAACARRTTGTTC

Forward/nuclear cox2 targeting sequence

6: CCAYCTCAGTAGTTATATTAT

Reverse/nuclear cox2 targeting sequence

7. CTTGAGACCTCTGGTGTCTT

Reverse/nuclear cox2 targeting sequence

8. TACCATTGATGYCCAATAGC

Reverse/mature coding region of nuclear cox2

9. CCAATACCATTGATGYCCAA

Reverse/mature coding region of nuclear cox2

10. MCCAGGTACWGCATCACNTT

Reverse/mature coding region of nuclear cox2

11. ACAGCTTCYACAACRATAGG

Reverse/mature coding region of nuclear cox2

12. AWACCCADGAASCATAATC

Reverse/mature coding region of nuclear cox2

13. GGGATTAKTKGAHTTGAYTG

Reverse/mature coding region of nuclear cox2

14. CCATACTACAAAACCCCCAG

Reverse/mature coding region of Amphicarpa nuclear cox2

15. GAGCTATCGTAGGCATCAAT

Reverse/mature coding region of Pachyrhizus nuclear cox2

16. CCCAACCAGCGATTTACAACAA

Forward/5' UTR of mitochondrial cox2

17. TTGTTGGGGGAAGCAGTTA

Forward/5' UTR of mitochondrial cox2

18. CCTACTCTTTCATTTCCGC

Reverse/3' UTR of mitochondrial cox2

UTR, untranslated region.