Neuronal overexpression of mutant amyloid precursor protein results in prominent deposition of cerebrovascular amyloid -- Data Supplement - -- Proceedings of the National Academy of Sciences

Supplementary material for Calhoun et al. (1999) Proc. Natl. Acad. Sci. USA 96 (24), 14088-14093.

Results
Vascular Amyloid Occurred in a Wide Range of Vessels and Covers a Significant Portion of the Vessel Surface.
To assess the biological significance of vascular amyloid, stereological techniques were used to estimate the percent of total vessel surface area covered with fibrillary amyloid in different brain regions of three mice with the highest CAA grades. Histological sections were immunostained for ß-dystroglycan and counterstained with Congo red to visualize amyloid containing vessels (supplemental Fig. 7 A-C). In addition, the inner diameter of sampled vessels was measured to give an indication of which vessels were more prone to develop pathology. Results, shown in Table 1 in the main text, indicated that a majority of pial vessels were affected, and a significant percentage of vessel surface area in thalamus, cortex, and hippocampus also contained fibrillary amyloid. The size distribution of affected vessels indicated that larger vessels were more likely to contain fibrillary amyloid, although vessels of all sizes, from capillaries to large arteries, were affected to some degree (supplemental Fig. 7D). Amyloid Deposition and High Cerebrospinal Fluid Aß Levels Are Also Present in APP23 Mice on an App-Null Background.
In APP23 mice on an App-null background [C57BL/6-App-TgN(Thy1-APPSwe)], prominent vascular amyloid deposition was also observed, and observations were indistinguishable from those for the APP23 mice alone (supplemental Fig. 8).