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Figure S2. Gating strategies in an eight-color flow cytometric analysis of lung samples. (A) Myeloid DCs were gated as live cells (CD45⁺ and low fluorescent). Next, CD11b⁺ and CD11b⁻ fractions were determined, and within these populations the MHCII⁺CD11c⁺ DCs were gated. (B) pDCs were gated as live cells (CD45⁺ in the lymphocyte gate). They were then gated as CD11b⁻MHCII^int cells expressing CD11c^int and high mPDCA-1. (C) Alveolar macrophages were determined in BAL fluid as highly autofluorescent cells expressing high levels of CD11c. The CD11b⁻ fraction was then gated for F4/80⁺ cells.