Supplementary material for Waters and Vierling (1999) Proc. Natl. Acad. Sci. USA 96 (25), 14394-14399.
Materials and Methods
Bacterial, Archaeal and non-land plant Eukaryotic sHSPs:
Aquifex aeolicus
, sHSP, AE000732; Archaeoglobus fulgidus
, HSP20-1, AE001014; Archaeoglobus fulgidus
, HSP20-2, AE000967; Azotobacter vinelandii
, HSP16, P96193; Bos taurus
(B. ta.), Alpha Crystallin A, M26142; Bradyrhizobium japonicum
, HSPC, U55047; Bradyrhizobium japonicum
, HSPF, AJ003064; Brugia pahangi
, sHSP, S57399; Caenorhabditis elegans
, HSP16-48, P02513; Caenorhabditis elegans
, HSP16-2, P06582; Caenorhabditis elegans
, HSP 16-1, P06581; Caenorhabditis elegans
, HSP20, U41109; Chlamydomonas reinhardtii
, HSP22, X15053; Drosophila melanogaster
(D. mel.), HSP23, P02516; Drosophila melanogaster
(D. mel.), HSP27, P02518; Emericella nidulans
, HSP30; P40920; Escherichia coli
, IbpA, M94104; Escherichia coli,
IbpB, M94104; Homo sapiens
(H. sa.), HSP27, P04792; Homo sapiens
(H. sa.) Alpha Crystalin A, P02489 Homo sapiens
(H. sa.), Alpha Crystallin B, P02511; Mesocricetus auratus
(M. aur.), Alpha Crystallin A, P02490; Methanobacterium thermoautotrophicum
, sHSP, AE000862; Methanococcus jannaschii
, HSP16; MJ0285; Mycobacterium avium
, HSP18, P46731; Mycobacterium intracellulare
, HSP18, P46732; Mycobacterium leprae
, HSP18, P12809; Mus musculus
(M. mus.), HSP25, U03560; Mus musculus
, Alpha crystallin B, M73741; Neurospora crassa
, HSP30; M55672; Onchocerca volvulus
, HSP25-2, S29693; Onchocerca volvulus
, HSP25-1; S29692; Oryctolagus cuniculu
s (O. cun.), Alpha Crystallin B, P41316 Rattus norvegicus
(R. nor.), HSP27, M86389; Rickettsia prowazekii
, sHSP, AJ235271; Saccharomyces cerevisiae
, HSP26, P15992; Sarcophaga crassipalpis
(S. crass.), HSP23, U96099; Schizosaccharomyces pombe
, HSP16-1, AJ003817; Schizosaccharomyces pombe
, HSP16-2, AL023781; Stigmatella aurantiaca, Q06823;
Streptomyces albus
, HSP18, Q53595; Synechococcus vulcanus
, HSPA, AB002666; Synechocystis sp
. PCC 6803, HSP16, D90902 Chloroplast sHSPs:
F. hygrometrica
, HSP 22; F. hygrometrica
, HSP 21; Lycopersicon esculentum
, HSP 20 , Q95661; Petunia hybrida
, HSP 21, P30222; Pisum sativum
, HSP 21, P09886; Arabidopsis thaliana
, HSP 21, P31170; Triticum aestivum
, HSP 26 A X58280; Hordeum vulgare
, HSP 26, S39498; Zea mays
, HSP 26, L28712 Mitochondrial sHSPs:
Pisum sativum
, HSP 22 MT, P46254; Glycine max
, HSP 23.9 MT, S65049; Chenopodium rubrum
, HSP 23 MT, P11890; Arabidopsis thaliana
,HSP 23.6 MT, U72958; Picea glauca
, HSP 23.5 MT, L47741 Plant Cytosolic I sHSPs,
Arabidopsis thaliana
,HSP 17.6 I, X16076; Arabidopsis thaliana
, HSP 17.4 I, X17293; Helianthus annuus
, HSP 17.6 I, X59701; Oryza sativa
, HSP 17.8 I, X75616; Pseudotsuga menziesii
, HSP 18.2A, X92983 Zea mays
, HSP 17.2 I, X65725; Funaria hygrometrica
, HSP 17.2 A I, AF087640; Funaria hygrometrica
, HSP 17.2 B I, AF089842; Funaria hygrometrica
, HSP 17.2 C I, AF089843; Funaria hygrometrica
, HSP 16.5 I, AF089844 Cytosolic II sHSPs:
Arabidopsis thaliana
, HSP 17.6 II, X63443; Funaria hygrometrica
, HSP 16.4 II, AF089845 ; Funaria hygrometrica
, HSP 18.3 II, AF089846; Helianthus annuus
, HSP 17.9, Z29554 ; Picea abies
, HSP 16.9 II, X99346; Picea glauca
, HSP 17.0 II, L47740 ; Pisum sativum
, HSP 17.7, M33901 ; Triticum aestivu
, HSP 17.3 Ii, X58279 ER sHSPs:
Arabidopsis thaliana
, HSP 22, U11501; Glycine max
, HSP 22.2, U21723; Pisum sativum
, HSP 22, X86222; Glycine max
, HSP 22.3, S65050
Alignment used in phylogenetic analysis:
Mycobacterium leprae
AWREG-EEFVVEFDLPGIK-ADSLDIDIERN-VVTVRAE-RPGVDPDR--------
EMLAAERPRGVFNRQLVLG----ENLDTERILASYQEGVLKLSIPVA-----ERAKPRKISVD-----------
Mycobacterium avium
AWRDG-DQFVVEFDLPGVN-ADSLDLDVERN-VLTVRAE-RPDLDQNR--------EMVSAERPRGVFSRQLFLD--
---NLDTDKIEANYHDGVLRLTIPVA-----EKAKPRRIEINHNHR--TAINA
Mycobacterium intracellulare
AWRDG-EQFVVEFDLPGID-EQSLDLDIERN-VVTVRAE-RPDVDPSR--------EMLATERARGVFSRQLVLG--
--DNLDTEHIDACYDAGVLRLRIPVA-----EKAKPRKIAVNRGDRQQTAISA
Stimgmatella aurantiaca
EVRETKEAYIFKADLPGVD-EKDIEVTLTGD-RVSVS-GKREREKREES------ERFYAYERTFGSFSRAFTLP--
--EGVDGDNVRADEKNGVLTLTLPKR-----PEVQPKRIQVASSGTEQKEHIK
Bradyrhizobium japonicum HSPC
DIDETDKEVRITAELPGLE-EKDVSLEIANG-VLSIS-GEKKSESEDK-------ARRF-SERYY--
FERR--IP---LEGIDEDKVSAAFKNGVLTITVPKS-----AEAK-NVRRIAINNRNG-----
Bradyrhizobium japonicum HSPF
EFNETDKEVKVIAELPGIE-QKDVEVELADG-VLTIS-GEKKSETKDK-------ERRF-SERYYGRFERR--IP--
-VDDVDQDKVAASFKDGILTVTLPKS-----PTEQRKVKRIAINGK-------
Rickettsia prowazekii
FITKDK-QYIIVMEVPGFD-KSQIKVKLNGK-KLFI-AGNIEDKNKAND----------SDNYMNKNFNYVISLY--
--EDVDQKSISARLKNGILTIILPRI-----EVKEQDSREITIN---------
Escherichia coli IpbB
VEKSDDNHYRITLALAGFR-QEDLEIQLEGT-RLSVK-GTPEQPKEEKK-----WLHQGLM---NQPFSLSFTLA--
-GENMEVSG--ATFVNGLLHIDLIRN-----EPEPIAAQRIAISERPALNS--
Streptomyces albus
DACREGDTYVVSFDLPGVD-PEAIEIDIERR-MLTVKAERGPAGNAEHVR-------MEVAERPLGVFSRALVLA--
--DTLDTEQVRADYDAGVLTLRIPIA-----ERAKRRRVKVGQGESHRQITG-
Azobacter vinelandii
-IEHGDERYRIVIAAAGFQ-ESDLDVQVERG-VLSVSGGKRENRSEGV-----TYLHQGIAQRAF-
KLS--FRLV----DHIEVKG--AELRNGLLSIELVRI-----VPERPA----------------
Synechococcus vulcanus
ELEETPEALLLKVELPGMD-PKDIDVQVTAE-AVSISGERKSETKTETE-------GMKRTEFRYGKFQRVIPLP--
-VRIQNTS-VKAEQKDGILHLTLPKA-----EEEKNRVVKVSLA---------
Escherichia coli IpbA
VELVDENHYRIAIAVAGFA-ESELEI--TAQ-DNLLVVKGAHADEQKER----TYLYQGIAERNF---ERKFQLA--
--ENIHVRG--ANLVNGLLYIDLERV-----IPEAKKPRRIEIN---------
Synechocystis sp.
ELTETEEAYVLKLELPGMD-PDNLDIQAARD-AVTVSGDRQDTHSTEKD-------GVRRTEFRYGSFRRVIPVP--
-GAIQNTE-VKANYDAGILTLTLPKV-----EEAKNKVVKVQLS---------
Methanobacterium thermoautotrophicum
DVMETDDAIIIKTDLPGVK-KEDINIELTEN-TISISAVFEEEVEIK-------EADFIKKERKYGEAKREMRLP--
--EKIRVEDASAKFENGVLTVELPKV-----EVKKKQTLKVE-----------
Aquifex aeolicus
EVYETDKEVVIKMDLPGVK-KEDIEITVKEN-AVHVRAERKEEKEEKT-------ENIIRAERFYGVIERVIPLP--
--VEVDPEKAEAKYENGVLEIRIPKV-----HVEKEKKIKLA-----------
Archaeoglobus fulgidus HSP 20-2
VIDEGEQ-IRVVADLPGFS-KEDLEIYFEDG-DLVIKAEKKEEFEEKKG------EYLRR-ERRMGKVYRRIALP--
--AGLDIDAVKAKYNNGVLEITIPKL-----KKDRKAVQIE------------
Methoanococcus jannaschii
SIIEGDQHIKVIAWLPGVN-KEDIILNAVGD-TLEIRAKRSTESER----------IIYSEIPEEEEIYRTIKLP--
--ATKE-ENASAKFENGVLSVILPKA-----ESSIKKGINIE-----------
Archeoglobus fulgidus HSP 20-1
DVIETDNEIQVIAEMPGVN-KDDIELNATET-TLEIRAEGENR-----------------------KYYETVELP--
--AEVDPDSAKARYNNGVLEVILKKK----TPKTSGKKIKIE-----------
Pisum sativum HSP 22 ER
DWKETPEGHVIMVDVPGLK-KDDIKIEVEENRVLRVSGERKKEEDK-------KGDHWHRVERSYGKFWRQFKLP--
-QNVDL-DSVKAKMENGVLTLTLHKS---HDKIKGPRMVSIVEE---------
Glycine max HSP22.2 ER
DWKETPEGHVIMLDVPGLK-REEIKVEVEENRVLRVSGERKKEEEK-------KGDHWHRVERSYGKFWRQFRLP--
-QNVDL-DSVKAKLENGVLTLTLDKS---PGKIKGPRVVSIAGE---------
Arabidopsis thaliana HSP 22 ER
DWKETAEGHEIMLDIPGLK-KDEVKIEVEENGVLRVSGERKREEEK-------KGDQWHRVERSYGKFWRQFKLP--
-DNVDM-ESVKAKLENGVLTINLTKS---PEKVKGPRVVNIAAE---------
Glycine max HSP 22.3
DWKETPSAHVIVLDLPGMK-KKDVKIEVEESRVLRISGERKGEEEEE-----VEGEKWHRAERTNGKFMRQFRLP--
-VNADL-EKVTARLENGVLRITVGKFG--EDKKRQPKVIDIAQ----------
Helianthus annuus HSP17.6I
DWKETPEAHVLKADLPGMK-KEEVKVEVED-RVLQISGERCREQEE-------KDDTWHRVERSSGKFIRRFRLP--
-ENAKM-DEVKAMMENGVLTVVVPKE---EEKKPMVKAIDISG----------
Pisum sativum HSP18.1I
DWKETPEAHVFKADLPGLK-KEEVKVEVEDDRVLQISGERSVEKED-------KNDEWHRVERSSGKFLRRFRLP--
-ENAKM-DKVKASMENGVLTVTVPKE---EIKKAEVKSIEISG----------
Zea mays HSP17.2I
DWKETPEAHVFKADLPGVK-KEEVKVEVEDGNVLVISGQRSREKED-------KDDKWHRVERSSGQFIRRFRLP--
-DDAKV-DQVKAGLENGVLTVTVPKA---EEKKPEVKAIEISG----------
Arabidopsis thaliana HSP 17.4I
DWRETPEAHVFKADVPGLK-KEEVKVEVEDGNILQISGERSSENEE-------KSDTWHRVERSSGKFMRRFRLP--
-ENAKV-EEVKASMENGVLSVTVPKV---QESKPEVKSIDISG----------
Arabidopsis thaliana HSP 17.6 I
DWRETPEAHVFKADLPGLR-KEEVKVEVEDGNILQISGERSNENEE-------KNDKWHRVERSSGKFTRRFRLP--
-ENAKM-EEIKASMENGVLSVTVPKV---PEKKPEVKSIDIS-----------
Oryza sativa HSP 18.7 I
DWKETPE-HVFKADVPGLK-KEEVKVEVEDGNVSRSAGEASKEQEE-------KTDKWHRVEASSGKFLRRFRLP--
-ENTKP-EQIKASMENGVLTVTVPKE---EPKKPDVKSIQVTG----------
Pseudotusga menziesii HSP 18.2 A
DWKETPEAHIFKADLPGLK-KEEVKIELEEGRILQISGERSKEEEQ-------KNDKWHRIERSRGKFLRRFRLP--
-DNAKV-EEIKAAMENGVLTVTVPKQP--EPQPPQPKSIEISG----------
Funaria hygrometrica HSP17.2A I
DWRETPEAHVFKADLPGLK-KEEVKVQVVEGRTLEISGERKKEEVQ-------KGDTWHRVERAQGSFMRRFRLP--
-EGTNV-DEVKAQVQDGVLTVTIPKL---QKPKPQVRQIEIA-----------
Funaria hygrometrica HSP17.2 BI
DWRETPEAHIFKADLPGLT-KEEVKVQVLEGRTLEICGERKKEEVQ-------KSDTWHRMERAQGSFMRRFRLP--
-EGTNT-DDVKAQVQDGVLTVTVPKV---QKPKPQVRQIEIA-----------
Funaria hygrometrica HSP 16.5 I
DWRETPEAQSSKADLPGLK-KEEVKVQLVDGKTLEISGERRKEEVH-------KDDTWHRVERAHGSFLRRFRLP--
-DNSNV-EAVEAQVQDGVLTVTIPKI---QKPKPQVRQIEIA-----------
Funaria hygrometrica HSP 17.2 CI
DWKETPTEHVIKADLPGLK-KEEVHVQVEGDRTLSISGQRKHEEVQ-------KTDTWHRVERSSGQFMRKFRLP--
-ENANL-EQISAQVQDGVLTVKIPKL---EKQKPHSRTIEIG-----------
Picea glauca HSP 17.0 II
DVKEYPNSYVFIVDMPGLK-SNDIKVQVEDENILNISGERKRNEKEE------GEVKYIRMERRVGKFMRKFSLP--
-ADCNL-EAISAACQDGVLTVTVPK-P--PPEPKKPKTIAVQIG---------
Picea abies HSP 16.9 II
DVKEYPNSYVFIVDMPGLK-SNDIKVQVEDENVLNISGERKRNEKEE------GEVKYIRMERRVAKFMRKFSLP--
-ADCNL-EAISAACQDGVLTVTVPKLP--PPEPKKPKTIAVKIG---------
Helianthus annuus HSP 17.9 II
DVKECPNSYVFIVDMPGLK-SGDIKVQVERDNVLVISGKRNREEE-K------EGVKYVRMERRMGKFMKKFALP--
-EDANT-DKISAICQDGVLTVTVEKLP--PPEPKKPKTIQVQVA---------
Pisum sativum HSP 17.7 II
DVKEHPNSYVFMVDMPGVK-SGDIKVQVEDENVLLISGERKREEE-K------EGVKYLKMERRIGKLMRKFVLP--
-ENANI-EAISAISQDGVLTVTVNKLP--PPEPKKPKTIQVKVA---------
Triticum aestivum HSP 17.3 II
DVKELPGAYAFVVDMPGLG-SGDIKVQVEDERVLVISGERRREE--K------EDAKYLRMERRMGKLMRKFVLP--
-ENADM-EKISP-CRDGVLTVTVDK-P--PPEPKKPKTIQVQVA---------
Arabidopsis thaliana HSP 17.6 II
DVIEHPNAYAFVVDMPGIK-GDEIKVQVENDNVLVVSGERQRENKEN------EGVKYVRMERRMGKFMRKFQLP--
-ENADL-DKISAVCHDGVLKVTVQK-P--PPEPKKPKTIQVQVA---------
Funaria hygrometrica HSP 16.4 II
DVKELPDAYIFVADMPGLK-SADVKVQLENDNVLVIGGTRKREEP-D------PKVKYIRMERNSGSFMRKFTLP--
-QNSNL-DKIAASCVNGILTVTVPK-P--PPEPAKPRTIEVTMG---------
Funaria hygrometrica HSP 18.3 II
DVKEIKDAYLFVADVPGLQ-KTDIEVQVENENVLTMRGKRKLEKVEKE-----EDTKFIRMERSPVKLLRKFTLP--
-SDANA-DAITANCVDGVLTVTVPK-P--PPEPAKSKTVQIAVD---------
Pisum sativum HSP 22 MT
DARETEDALFLRLDMPGLG-KEDVKISVEQ-NTLTIKGEE-GAKESEEK------------EKSGRRFSSRIDLP--
-EKLYKIDVIKAEMKNGVLKVTVPKMK--EEERNNVINVK-------------
Glycine max HSP23.9 MT
DARETEDALHLRVDMPGLA-KEDVKISVEQ-NTLIIKGE--GAKEGDE-------------EESARRYTSRIDLP--
-DKLYKIDQIRAEMKNGVLKVVVPKMK--EEERKDVISVKVE-----------
Chenopodium rubrum HSP 23 MT
DVREDEEALELKVDMPGLA-KEDVKVSVED-NTLIIKSE--AEKETEE-------------EEQRRRYSSRIELT--
-PNLYKIDGIKAEMKNGVLKVTVPKIK--EEEKKDVFQVMV------------
Picea glauca HSP 23.5 MT
DAVEDKEALHLRVDMPGL--KEDIKVYAEE-NALVIKGE---SVSDAEL------------DGSARKYSSRIELP--
-PKVYKLDHIKAQMKNGVLKVTVPKFT--EQEIKNVINVNI------------
Arabidopsis thaliana HSP 23.6 MT
DIKEKDDALYLRIDMPGLS-REDVKLALEQ-DTLVIRGEGKNEDGGEEG------------ESGNRRFTSRIGLP--
-DKIYKIDEIKAEMKNGVLKVVIPKMK--EQERNDVRQIEIN-----------
Petunia hybrida HSP 21
DIKDDENEIKMRFDMPGLS-KEEVKVSVED-DVLVIKGEHKKEESGKDDS-------W---GRNYSSYDTRLSLP--
-DNVDK-DKVKAELKNGVLLISIPKTK----VEKKVTDVEIK-----------
Arabidopsis thaliana HSP21 CP
DIKEEEHEIKMRFDMPGLS-KEDVKISVED-NVLVIKGEQKKEDSDS----------WS--GRSVSSYGTRLQLP--
-DNCEK-DKIKAELKNGVLFITIPKTK----VERKVIDVQIQ-----------
Lycoperiscon esculentum HSP 20 CP
DIHDDENEIKMRFDMPGLS-KEDVKVSVEN-DMLVIKGEHKKEEDGRDKH-------W---GRNYSSYDTRLSLP--
-DNVVK-DKIKAELKNGVLFISIPKTE----VEKKVIDVQIN-----------
Hordeum vulgare HSP26 CP
DIMEDDKEVKMRFDMPGLS-REEVKVMVED-DALVIRGEHKKEAGEGQGGG-----DWW-KERSVSSYDMRLALP--
-DECDK-SQVRAELKNGVLLVSVPKRE----TERKVIDVQVQ-----------
Pisum sativum HSP 21CP
EIKDEEHEIRMRFDMPGVSKEDVKVSVEDDVLVIKSDHREE----------NGGEDCW--SRKSYSCYDTRLKLP--
-DNCEK-EKVKAELKDGVLYITIPKTK----IERTVIDVQIQ-----------
Triticum aestivum HSP 26A
DIMEDEKEVKMRFDMPGLS-REEVRVMVED-DALVIRGEHKKEAGEGQGGG-----DWW-KERSVSSYDMRLALP--
-DECDK-SQVRAELKNGVLLVSVPKRE----TERKVIDVQVQ-----------
Zea mays HSP 26 CP
DIVEDEKEVKMRIDMPGLA-RDEVKVMVED-DTLVIRGEHKKEEGAEGGSG-----DWW-KQRSVSSYDMRLALP--
-DECDK-SKVRAELKNGVLLVTVPKTE----VERKVIDVQVQ-----------
Funaria hygrometrica HSP22 CP
DVKEDADAYKLRFDMPGLS-KEEVKVSVED-GDLVIRGEHNAEDQKEDS--------WS--SRSYGSYNTRMALP--
-EDALF-EDIKAELKNGVLYVVVPKSK--KDAQKKVLDINVQ-----------
Funaria hygrometrica HSP 21 CP
DVKEDNESFRLGFDMPGLG-KDEVKVYVED-GDLVIKGAHRAEEQKENN--------WS--SRSYGSYNTRMTLP--
-ENVKI-DEVKAELKNGVLQVVVPKSK---EEKKNVIDINVE-----------
Caenorhabditis elegans HSp16-48
EIVNDESKFSVQLDVSHFK-PEDLKIELDG-RELKIEGIQEKKSE---------------HGYSKRSFSKMILLP--
-EDVDLTSVKSAISNEGKLQIEAPKKTNSS------RSIPINFVAKH------
Caenorhabditis elegans HSP 20
DVVNNDQEYNVSVDVSQFE-PEELKVNIVD-NQLIIEGKHNEKTD--------------KYGQVERHFVRKYNLP--
-TGVRPEQIKSELSNNGVLTVKYEKN-----QEQQPKSIPITIVPKR------
Caenorhabditis elegans HSP 16-1
EIVNDESKFSVQLDVSHFK-PENLKIKLDG-RELKIEGIQETKSE---------------HGYLKRSFSKMILLP--
-EDADLPSVKSAISNEGKLQIEAPKKTNSS------RSIPINFVAKH------
Caenorhabditis elegans HSP 16-2
EIVNNDQKFAINLNVSQFK-PEDLKINLDG-RTLSIQGEQELKTD---------------HGYSKKSFSRVILLP--
-EDVDVGAVASNLSEDGKLSIEAPKKEAVQG-----RSIPIQQAIVEEKSAE-
Emericells nidulans HSP30
DVRESNEAYHLDGELPGI--PQSNIDIEFT-DPLVIKGRSEREYHSSSDQVKKAANKYWVSERSVGEFQRTRVNQ--
------DDVKASLK-DGILSLVVPKA-----VPPTAKKITIQ-----------
Neurospora crassa HSP30
DVRETEQTYELHGELPGI--DRDNVQIEFT-DPIVIRGRVERNTEEKP---KAPAEKYWVSERSIGEFSRTFNFP--
--------GRVSLN-NGILTITVPKA-----KKHETIRIAIN-----------
Schizosaccharomyces pombe HSP 16-2
ELRETEDTIEVDVELPGID-KQNLKVDLHG-SKLTISGERKKPEEEK-----AGPLIRW-SERCVGAFSRTITLP--
-QPVDEKLIHASLN-NGILSIVMKKKNP----EFTTRIVEIQ-----------
Chlamydomonas reinhardtii HSP22
DIIESPTAFELHADAPGMG-PDDVKVELQG--VLMVTGERKLSHTTK----EAGG-KVWRSERTAYSFSRAFSLP--
-ENANPDGITAAMD-KGVLVVTVPKREP--PAKPEPKRIAVTGA---------
Saccharomyces cerevisiae HSP26
DILDHDNNYELKVVVPGVKSKKDIDIEYHQKNQILVSGELNEESKD----------KVKVKESSSGKFKRVITLP-
DYPGVDADNIKADYA-NGVLTLTVPKLKPQKDGKNHVKKIEVSSQESWGN----
Schizosaccharomyces pombe HSP16-1
DVHEGKDTVSVDVELPGVK-KEDVQVHYDS-GKLTISGEVVNERKNE----STEGNQRW-SERRFGSFSRTITIP--
-AKIDADRIEANFS-NGLLTVTLPKVE----KSQTKKQIAIK-----------
Mus musculus HSP25
EIRQTAD-WRVSLDVNHVI-PEELTVKTKE-GVVEITGKHEERQD--------------EHGYISRCFTRKYTLP--
-PGVDPTLVSSSLSPEGTLTVEAPLPKAVTQS--TEITIPVTFE---------
Brugia pahangi
EVINEKDKFAIQVDVSHFH-PKELSVSVRD-RELSIEGHHKERND------------HSGHGSIERHFVRKYVMP--
-EEVQTDTIESHLSDKGVLTICATKTMIGLP---AARNIPIR-----------
Onchocerca volvulus 25-2
EVIE-KDKFAVRADVSHFH-PKELSVSVRD-RELVIEGHHEERTDPA------------GHGSIERHFIRKYVLP--
-EEVQPDTIESHLSDKGVLTISANKT--AIGTT-ASRNIPIR-----------
Onchocera volvulus 25-1
EVINEKDKFAVRADVSHFH-PKELSVSVRD-RELVIEGHHKERADSA------------GHGSIERHFIRKYVLP--
-EEVQPDTIESHLSDKGVLTICANKT--AVGTT-ASRNIPIRASPKEPEAKQK
Sarcophaga crassipalpis HSP23
SPV-GKDGFQVCMDVAQFK-PSELNVKVVD-NCIVIEGKHEERED--------------QHGFIQRHFVRRYVLP--
-KGYDADKVVSTLSSDGVLTVSVPKP-QEEEKS-NERVIQIQQTGPAHLNVKQ
Drosophila melanogaster HSP23
SKI-GKDGFQVCMDVSHFK-PSELVVKVQD-NSVLVEGNHEERED--------------DHGFITRHFVRRYALP--
-PGYEADKVASTLSSDGVLTIKVPKPPAIEDKG-NERIVQIQQ----------
Drosophila melanogaster HSP27
PAV-GKDGFQVCMDVSQFK-PNELTVKVVD-NTVVVEGKHEERED--------------GHGMIQRHFVRKYTLP--
-KGFDPNEVVSTVSSDGVLTLKAPPPPSKEQAK-SERIVQIQQ----------
Mus musculus Alpha-B
EMRLEKDRFSVNLDVKHFS-PEELKVKVLG-DVIEVHGKHEERQD--------------EHGFISREFHRKYRIP--
-ADVDPLTITSSLSSDGVLTVNGPRKQVSG----PERTIPITREEK-------
Oryctolagus cuniculus Alpha-B
ERMLEKDRFSVNLDVKHFS-PEELKVKVLG-DVIEVHGKHEERQD--------------EHGFISREFHRKYRIP--
-ADVDPLTITSSLSSDGVLTVNGPRKQAPG----PERTIPITREEK-------
Homo sapiens Alpha-B
EMRLEKDRFSVNLDVKHFS-PEELKVKVLG-DVIEVHGKHEERQD--------------EHGFISREFHRKYRIP---
ADVDPLTITSSLSSDGVLTVNGPRKQVSG----PERTIPITREEK
Rattus norvegicus CAA24530
EVRSDRDKFVIFLDVKHFS-PEDLTVKVLE-DFVEIHGKHNERQD--------------DHGYISREFHRRYRLP--
-SNVDQSALSCSLSADGMLTFSGPKVQSGLDAGHSERAIPVSREEKPSSAPSS
Bos taurus Alpha-A
EVRSDRDKFVIFLDVKHFS-PEDLTVKVQE-DFVEIHGKHNERQD--------------DHGYISREFHRRYRLP--
-SNVDQSALSCSLSADGMLTFSGPKIPSGVDAGHSERAIPVS-----------
Homo sapiens Alpha-A
EVRSDRDKFVIFLDVKHFS-PEDLTVKVQD-DFVEIHGKHNERQD--------------DHGYISREFHRRYRLP--
-SNV-QSALSCSLSADGMLTC-GPKIQTGLDATHAERAIPVSREEKPTSAPSS
Rattus norvegicus HSP27
EIRQTADRWRVSLDVNHFA-PEELTVKTKE-GVVEITGKHEERQD--------------EHGYISRCFTRKYTLP--
-PGVDPTLVSSSLSPEGTLTVEAPLPKAVTQS--AEITIPVTF----------
Homo sapiens HSP27
EIRHTADRWRVSLDVNHFA-PDELTVKTKD-GVVEITGKHEERQD--------------EHGYISRCFTRKYTLP--
-PGVDPTQVSSSLSPEGTLTVEAPMPKLATQS--NEITIPVTFE---------