The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome -- Hansen et al. 96 (25): 14412 Data Supplement - Supplemental Table -- Proceedings of the National Academy of Sciences

Supplementary material for Hansen et al. (1999) Proc. Natl. Acad. Sci. USA 96 (25), 14412-14417.

A correction to this Supplemental Table has been posted.

Table 2.
Human DNMT3B primer pairs cDNA amplimers

Name	Sequence (5' to 3')	5' position	Size, bp		Comments
cDNA amplimers
mt3b-2f mt3b-2r	AGGAAAGCATGAAGGGAGACACC TCGCCTGTCAAGTCCTGTGTGTA	Exon 0 Exon 3		226
mt3b-3f mt3b-3r	GGAAGACTCGATCCTCGTCAAC CGGCTGGAGACACTGTTGTTATT	Exon 1 Exon 4		291
mt3b-4f mt3b-4r	AGGGAGGTGTCCAGTCTGCTAAG GTCCTCTGTGTCGTCTGTGAGGT	Exon 2 Exon 5		357
mt3b-5f mt3b-5r	GCTGTCCGAACTCGAAATAACAA CTGTCTCCATCTCCACTGTCTGC	Exon 3 Exon 5		230?, 338	alternative splice for exon 4? (minus exon 4-sized product)
mt3b-6f mt3b-6r	ACCTCACAGACGACACAGAGGAC TCTGCAGAGACCTCGGAGAACTT	Exon 5 Exon 7		319
mt3b-7f mt3b-7r	AGGAGTTTGGAATAGGGGACCTC CAGCTGGTCCTCCAATGAGTCT	Exon 6 Exon 8		326
mt3b-8f mt3b-8r	ATTTGGCCACCTTCAATAAGCTC AAtACAGCTCAAGGAAGCGATCC	Exon 7 Exon 13		477, 537	alternative splice: exon 9
mt3b-9f mt3b-9r	AACAAGACTCGAAGACGCACAG CTTCCAGGCTGCTCTTGTTGTT	Exon 10 Exon 11		169
mt3b-10f mt3b-10r	GAACAAATGGCTTCAGATGTTGC CAAACAATGACAGGACTCGAATG	Exon 10 Exon 15		493	use r primer for sequencing, has exon 14 c/t polymorphism
mt3b-11f mt3b-11r	CCCTGGAGCTGTTACATGTGTCT TTCCTCACGTCGTTCACGTATTT	Exon 14 Exon 16		329
mt3b-12f mt3b-12r	CGCTTCTGAAGTGTGTGAGGAGT GTGAGCAGCAGAAACTTTGATGG	Exon 16 Exon 19		385
mt3b-13f mt3b-13r	CCATGCAACGATCTCTCAAATGT GTTTGATCGAGTTCGACTTGGTG	Exon 17 Exon 21		330, 400	alternative splice: exon 21
mt3b-14f mt3b-14r	CCTGGAGTGTAATCCAGTGATGA GTAGTGCACAGGAAAGCCAAAGA	Exon 18 Exon 22		118, 188, 237, 307	alt. splices: exons 20 or 21
mt3b-14f mt3b-15r	CCTGGAGTGTAATCCAGTGATGA ACATGCAAAGTAGTCCTTCAGAGG	Exon 18 Exon 22		229, 299, 348, 418	alt. splices: exons 20 or 21
mt3b-16f mt3b-16r	CCCGTGATAGCATCAAAGAATGA GCCTTCAGGAATCACACCTCCT	Exon 20 Exon 22		282, 401	alt. splice: exon 20
mt3b-17f mt3b-17r	TCGCCCCTCTGAAGGACTACTTT CACAGAGCAGTAGCCAGTTTTCAA	Exon 22 Exon 22		484	DNA amplimer also 484 bp
DNA amplimers
mt3b-1f mt3b-1r	GCTAATTGCACAGAGCAGTCTGA AAGCCTCCAGTTGTCTGCAGTG	Intron 0 Intron 1		523	first coding exon
mt3b-18f mt3b-18r	TGTTTTGTTTTGTTTTCCCCTCA CCAACGTTAACCTGGAGGGTATT	Intron 8 Intron 9		228	alternative splice: exon 9
mt3b-19f mt3b-19r	ATAGCAGGGAGTGGAGGAAATGA CTACATGTGGGTTTCAGGGTGTG	Intron 15 Intron 16		303	specific mutation screening with Tsp45I
mt3b-20f mt3b-20r	CCTGATGGCAGTAGGTCTTTCCT TCCATCAGCAGCTTACAACAGGT	Intron 18 Intron 19		453
mt3b-21f mt3b-21r	CTCATGCCAGGATCATTTTCATC GTTGCCTTTTCTCGTCGTGATTA	Intron 19 Intron 20		430
3bx19gt-f mt3b-20r	CAGTGATGATTGATcCtATCAAAG TCCATCAGCAGCTTACAACAGGT	Exon 19 Intron 19		304	f primer altered for specific mutation screening with EcoNI
mt3b-22f mt3b-22r	TGTCTCTCCAACTCTGCTTTTCG CAGTAACACCAAAGGGCAGTGAG	Intron 20 Intron 21		449
mt3b-23f mt3b-23r	TCCTCTGAGCACCAACTCTATGC GCCTTCAGGAATCACACCTCCT	Intron 21 Exon 22		457	specific mutation screening with HpaII