Biochemical and Mutational Analyses of AcuA, the Acetyltransferase Enzyme That Controls the Activity of the Acetyl Coenzyme A Synthetase (AcsA) in Bacillus subtilis
J. Bacteriol. Gardner and Escalante-Semerena 190: 5132 Supplemental material
Files in this Data Supplement:
- Supplemental file 1 - Supplemental materials and methods; Fig. S4, optimization of the AcuA activity assay; Fig. S5, kinetics of the reactions catalyzed by wild-type and variant AcuA proteins; Fig. S6, sequence comparisons between AcuABs and AcuAEs; Fig. S7, putative structural effects of mutations on AcuA proteins; Table S2, strains and plasmids.
PDF file, 1MB.