From the Cover: An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation -- Data Supplement - -- Proceedings of the National Academy of Sciences

Supplementary material for Sharp and Pogliano (1999) Proc. Natl. Acad. Sci. USA 96 (25), 14553-14558.

B. Scoring of engulfment phenotypes of several spoIIIE mutants

Strain	Wild type		spoIIIE ::spc		spoIIIE73-11		spoIIIE36
Time of sporulation, hr	3	4	3	4	3	4	3	4
Flat polar septa	121	54	184	129	131	30	102	36
Engulfing¹	59	27	166	203	76	15	48	70
Membrane migration complete²	68	41	115	237	259	100	250	335
Engulfment complete³	220	394	4	2	130	130	22	77
Dispores⁴	5	20	66	76	10	5	20	7
Not sporulating	463	552	570	447	678	311	360	390
Total sporangia	473	536	535	647	602	280	442	525
Total cells	936	1,088	1,105	1,094	1,280	591	802	915

% polar septa	26	10	34	20	22	11	23	7
% engulfing	12	5	31	31	13	5	11	13
% complete migration	14	8	21	37	43	36	57	64
% complete engulfment	47	74	0.7	0.3	21	46	5	15
% sporulation	51	49	48	59	47	47	55	57

Fig. 5.
(A) Plot showing the percentage of wild-type sporangia that have completed membrane migration (squares) or membrane fusion (triangles) versus time after the initiation of sporulation by resuspension. Samples were harvested every 15 min starting at t_1.5, and at least 300 sporangia were scored from each time point. (B) Scoring of different engulfment phenotypes of spoIIIE::spc, spoIIIE73-11, and spoIIIE36 at 3 or 4 hr after the initiation of sporulation. ¹Sporangia in the process of engulfment. ²Sporangia in which membrane migration is complete, but fusion has not yet occurred. ³Sporangia that have completed membrane movement and fusion. ⁴Sporangia with partial or complete septa at both poles.