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Figure S2. Chromosomally tagged Atg proteins had a more consistent expression level from cell to cell than proteins expressed from centromeric plasmids. (A) Representative images of chromosomally expressed or plasmid-based GFP-Atg8. GFP-Atg8 cells (YZX247) or wild-type cells expressing pGFP-Atg8(416) were grown in nutrient-rich medium and imaged by microscopy. Bar, 15 µm. (B) The signal intensity per cell was quantified from 50 cells of each strain and the relative SD from cell to cell was calculated. Relative SD is equal to SD divided by the mean. All cells expressed chromosomally tagged proteins except for those shown in lane 1, which expressed GFP-Atg8 from a centromeric plasmid.