PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein -- Data Supplement - Supplemental Figure -- Proceedings of the National Academy of Sciences

Supplementary material for Zheng et al., (1998) Proc. Natl. Acad. Sci. USA 95(25), 14745-14750.

Fig. 1 (supp.).
Analysis of PAT1 sequence with use of Lasergene (DNAStar, Madison, WI). (c) Sequence alignment of PAT1 with h-KLC (human kinesin light chain; GenBank accession no. Q07866) and C-KLC (Caenorhabditis elega ns; accession no. P46822) with use of the Clustal method with a residue weight of PAM250, and a gap penalty and gap length penalty of 10. Identical amino acids are shown in dark boxes, and conserved residues are shown in shaded boxes; hyphens indicate gaps. (d) Predicted domain structures of PAT1, h-KLC, and D-KLC (Drosophila). (e) Region of heptad repeat sequence found from Val-244 to Asp-278. There are seven heptad repeats in which core residues (a an d d) are enriched in hydrophobic residues, whereas salt bridges are predicted to form between positions e and g. (f) The phylogenetic tree (DNAStar, Madison, WI) of PAT1, h-KLC, r-KLC (rat, accession no. 11 1904), D-KLC, and C-KLC.