PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein -- Data Supplement - Supplemental Figure -- Proceedings of the National Academy of Sciences

Supplementary material for Zheng et al. (1998) Proc. Natl. Acad. Sci. USA 95(25), 14745-14750.

Fig. 4 (supp.).
Specificity of mAb26. (g) Western blot. Lysate from HeLa cells (lane 1) or from FLAG-PAT1-transfected COS-1 cells (lane 2) and purified recombinant PAT1 (lane 3) were separated by SDS/PAGE, and Western blo ts were probed with mAb26 hybridoma supernatant (dilution, 1:5) and detected by alkaline phosphatase reaction. mAb26 recognized a single predominant band of expected molecular weight from the HeLa lysate and transfected COS-1 lysates. The purified recombi nant PAT1 is of smaller size because it lacks the first 32 amino acids (lane 3). Longer color development reveals a closely spaced doublet of bands in HeLa and COS-1 lysates. (h) Paraformaldehyde-fixed HeLa cells were labeled with mAb8 (anti-GST an tibody) and visualized by use of the TSA system with HRP-conjugated secondary antibody and Cy3-tyramide.