Signal Transducer and Activator of Transcription-3 Is Required in Hypothalamic Agouti-Related Protein/Neuropeptide Y Neurons for Normal Energy Homeostasis
Endocrinology Gong et al. 149: 3346 Supplemental Data
Supplemental Data
Files in this Data Supplement:
- Supplemental Data - Supplemental Data
- Supplemental Figure 1 - Body weights of 20-week old mice of the indicated genotypes. Statistical analysis was performed by one-way ANOVA and the differences between means were determined by the Neuman-Keuls test. ** = statistically different from Stat3flox/flox and Stat3flox/- mice at the p
- Supplemental Figure 2 - Fluorescence in-situ hybridization (FISH) of metaphase chromosome spreads from spleen fibroblasts of AgrpCre breeders used to generate AgrpCre mice used in the study. Each diploid metaphase exhibited positive FISH signals (see arrows) close to the end of a pair of chromosomes and at the middle of one specific chromosome, consistent with hybridization to the endogenous Agrp loci near the end of chromosome 6 and the single integration site of the BAC transgene.
- Supplemental Figure 3 - Immunostaining of Egfp in the arcuate nucleus of AgrpCre/ROSA26Egfp mice. Staining was performed as described in Methods and was specific to the arcuate nucleus. Scale bar is 1.0 mm.