Supplementary material for Eisen et al. (1998) Proc. Natl. Acad. Sci. USA 95(25), 14863-14868.
Yeast experiments clustered in Fig. 2.
Experiment ID | Experiment description | Reference sample | Time, min | Ref. |
alpha 0 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 0 | 1 |
alpha 7 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 7 | 1 |
alpha 14 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 14 | 1 |
alpha 21 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 21 | 1 |
alpha 28 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 28 | 1 |
alpha 35 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 35 | 1 |
alpha 42 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 42 | 1 |
alpha 49 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 49 | 1 |
alpha 56 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 56 | 1 |
alpha 63 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 63 | 1 |
alpha 70 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 70 | 1 |
alpha 77 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 77 | 1 |
alpha 84 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 84 | 1 |
alpha 91 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 91 | 1 |
alpha 98 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 98 | 1 |
alpha 105 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 105 | 1 |
alpha 112 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 112 | 1 |
alpha 119 | Cell-cycle: Alpha factor arrest and release | Unsynchronized culture | 119 | 1 |
Elu 0 | Cell-cycle: Elutriation | Unsynchronized culture | 0 | 1 |
Elu 30 | Cell-cycle: Elutriation | Unsynchronized culture | 30 | 1 |
Elu 60 | Cell-cycle: Elutriation | Unsynchronized culture | 60 | 1 |
Elu 90 | Cell-cycle: Elutriation | Unsynchronized culture | 90 | 1 |
Elu 120 | Cell-cycle: Elutriation | Unsynchronized culture | 120 | 1 |
Elu 150 | Cell-cycle: Elutriation | Unsynchronized culture | 150 | 1 |
Elu 180 | Cell-cycle: Elutriation | Unsynchronized culture | 180 | 1 |
Elu 210 | Cell-cycle: Elutriation | Unsynchronized culture | 210 | 1 |
Elu 240 | Cell-cycle: Elutriation | Unsynchronized culture | 240 | 1 |
Elu 270 | Cell-cycle: Elutriation | Unsynchronized culture | 270 | 1 |
Elu 300 | Cell-cycle: Elutriation | Unsynchronized culture | 300 | 1 |
Elu 330 | Cell-cycle: Elutriation | Unsynchronized culture | 330 | 1 |
Elu 360 | Cell-cycle: Elutriation | Unsynchronized culture | 360 | 1 |
Elu 390 | Cell-cycle: Elutriation | Unsynchronized culture | 390 | 1 |
cdc15 10 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 10 | 1 |
cdc15 30 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 30 | 1 |
cdc15 50 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 50 | 1 |
cdc15 70 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 70 | 1 |
cdc15 90 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 90 | 1 |
cdc15 110 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 110 | 1 |
cdc15 130 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 130 | 1 |
cdc15 150 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 150 | 1 |
cdc15 170 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 170 | 1 |
cdc15 190 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 190 | 1 |
cdc15 210 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 210 | 1 |
cdc15 230 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 230 | 1 |
cdc15 250 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 250 | 1 |
cdc15 270 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 270 | 1 |
cdc15 290 | Cell-cycle: cdc15 arrest and release | Unsynchronized culture | 290 | 1 |
spo 0 | Sporulation | Time 0 | 0 | 2 |
spo 2 | Sporulation | Time 0 | 120 | 2 |
spo 5 | Sporulation | Time 0 | 300 | 2 |
spo 7 | Sporulation | Time 0 | 420 | 2 |
spo 9 | Sporulation | Time 0 | 540 | 2 |
spo 11 | Sporulation | Time 0 | 660 | 2 |
spo5 2 | Sporulation | 5-hr timepoint | 120 | 2 |
spo5 7 | Sporulation | 5-hr timepoint | 420 | 2 |
spo5 11 | Sporulation | 5-hr timepoint | 660 | 2 |
spo- early | Sporulation in ndt80 knockout | 2-hr timepoint | 120 | 2 |
spo- mid | Sporulation in ndt80 knockout | 5-hr timepoint | 300 | 2 |
heat 0 | Heat Shock 25-37C | Time 0 | 0 | |
heat 10 | Heat Shock 25-37C | Time 0 | 10 | |
heat 20 | Heat Shock 25-37C | Time 0 | 20 | |
heat 40 | Heat Shock 25-37C | Time 0 | 40 | |
heat 80 | Heat Shock 25-37C | Time 0 | 80 | |
heat 160 | Heat Shock 25-37C | Time 0 | 160 | |
dtt 15 | DTT Shock 1mm | Time 0 | 15 | |
dtt 30 | DTT Shock 1mm | Time 0 | 30 | |
dtt 60 | DTT Shock 1mm | Time 0 | 60 | |
dtt 120 | DTT Shock 1mm | Time 0 | 120 | |
cold 0 | Cold Shock 25-11 | Time 0 | 0 | |
cold 20 | Cold Shock 25-12 | Time 0 | 20 | |
cold 40 | Cold Shock 25-13 | Time 0 | 40 | |
cold 160 | Cold Shock 25-14 | Time 0 | 160 | |
diau a | Diauxic Shift | Time 0 | 540 | 3 |
diau b | Diauxic Shift | Time 0 | 660 | 3 |
diau c | Diauxic Shift | Time 0 | 780 | 3 |
diau d | Diauxic Shift | Time 0 | 900 | 3 |
diau e | Diauxic Shift | Time 0 | 1020 | 3 |
diau f | Diauxic Shift | Time 0 | 1140 | 3 |
diau g | Diauxic Shift | Time 0 | 1260 | 3 |
1. Spellman, P. T., Sherlock, G., Iyer, V. R., Zhang, M., Anders, K., Eisen, M. B., Brown, P. O., Botstein, D. & Futcher, B. (1998) Mol. Biol. Cell, in press.
2. Chu, S., DeRisi, J., Eisen, M., Mulholland, J., Botstein, D., Brown, P. O. & Herskowitz, I. (1998) Science 282, 699-705.
3. DeRisi, J. L., Iyer, V. R. & Brown, P. O. (1997) Science 278, 680-686.