Major recent and independent changes in levels and patterns of expression have occurred at the b gene, a regulatory locus in maize -- Data Supplement - -- Proceedings of the National Academy of Sciences

Supplementary material for Selinger and Chandler (1999) Proc. Natl. Acad. Sci. USA 96 (26), 15007-15012.

	`450`	`465`	`480`	`495`
`Mako`	`GTTCTTCGTCTACTG`	`GTTCTCAAATTGGTA`	`TTGCTTTTACCGGTG`	`GCAACTGCGAGTGTT`
`Zm cyp71`	`GATATGGTTTATTTG`	`ATTCTCAAATTGGTA`	`TTGCTTTTGCCTGTG`	`GCGACAGCAAGTGTT`
	`* * * * **`	`**************`	`****** ***`	` ****`

	`510`	`525`	`540`	`555`
`Mako`	`GAAAGGGTATTTTCT`	`GCATTGGTTATAGTG`	`AAATCAAAATCAAGA`	`AATAAGCTAGGTGAT`
`Zm cyp71`	`GAAAGGGCATTTTCT`	`GCAATGAGTTTGGTG`	`AAGACTAAATTGAGA`	`AACAAGATGTGTGAT`
	`***** *****`	`* * * ***`	`** * ** *`	` * * *****`

	`570`	`585`	`600`	`615`
`Mako`	`ACTATTTTAGATGAT`	`TGTCTACTCACGTTT`	`ATTGAGCGAGATATT`	`TTCTTCTAAGTTGAT`
`Zm cyp71`	`AGTCTTTTGGATGAT`	`TGTCTTGTTACATAC`	`ATCGAGAGGGATATT`	`TTCTTCGAAGTGAAT`
	`* * ** ****`	`***** * ** *`	` * * ******`	`**** `

	`630`	`645`	`660`	`675`
`Mako`	`GAAGGTGATATAATT`	`GAGTCATTCATGTCA`	`TTAGAAAAGCGGAGG`	`ATCAACAAGTAATAT`
`Zm cyp71`	`GAAGAAGATATAATT`	`GAAACTTTTATGGCC`	`TTGAGGAAGCGCAGG`	`CCAGATAAGTAGTAT`
	`** *******`	`** * * *`	` * *`	`* *** *`

	`690`	`705`	`720`	`735`
`Mako`	`TGTAAGTCTCTTCTA`	`TAGCTATACTTTAAA`	`TTATATGTATTTCAT`	`TTGAACAATTTGTAT`
`Zm cyp71`	`T-------------A`	`TTGCAAT-CTTTATG`	`TTTAATATATGCAAT`	`TTTCA-----TGCTT`
	`* *`	`* *****`	` * `	`** * ** *`

	`750`	`765`	`780`	`795`
`Mako`	`CAAGATTTATCGTCG`	`TACTCTATATAGTTT`	`TACCGAATTAGAATG`	`TGGTTTTACCG---A`
`Zm cyp71`	`ATTTGTTGAGTGTCA`	`AACTTTATATCTATA`	`TATCAAATTATGTAC`	`CAAATTTACTATGAA`
`Coix dapA`				`ATTTTACCA---A`
	`** * ***`	`* *** *`	`** * *****`	`..****.. `

	`810`	`825`	`840`	`855`
`Mako`	`ATT--ATTAATGGTT`	`TACCAAATTGTATAA`	`GACAAATTTTCCATC`	`GCATACCCTGTGATC`
`Zm cyp71`	`ATTATATGTAAATTA`	`GATAGAATTCTACAT`	`AAAAATTTTGTTGTG`	`GCACACCCTATGTAA`
`Coix dapA`	`ATCGGAATATGATTT`	`TATCGACTTATATAA`	`AAAAAATTGTCC--G`	`GCATATCCTGTGATA`
`Mako-rev`				`GCATACCCAG----C`
	`*. .... ..*.`	`..... .*.`	`....... .`	`**.......`
				`[ Terminal`

	`870`	`885`
`Mako`	`AAATCCTAGG--TTC`	`GCCACTGGT-ATTAC`	`T`
`Zm cyp71`	`AAATCCTAGG--TCC`	`GCCACTGACTCCGAC`	`T`
`Coix dapA`	`AAATCCTAGG--TCC`	`ACCACTGCCCATTAG`	`T`
`Mako-rev`	`ATATCCTGTGCATCC`	`GCCACTG`
	`.***.. ***`	`.****** . ...*.`	`*`
	`Inverted Repeat`	`]{Flankin`	`g}`

Fig. 8. Alignment of the Mako element and homologous sequences. The numbering is based on the entire Mako sequence and the direct repeat flanking it. Only the Mako sequence that is homologous to cyp71 and dapA is shown. The Mako-rev sequence is the reverse complement of the other terminal inverted repeat. The Coix lacryma-jobi dapA sequence (GenBank accession no. U61730) is part of intron 1 of that gene. The Zea mays cyp71c1 sequence (GenBank accession no. X81828) is from the upstream region, 462 bp upstream of the start of transcription. The "." and "*" shown below the sequence indicate 50-75% conservation and 100% conservation, respectively. The terminal inverted repeat sequence and 8-9 bp of flanking sequence are indicated.