Supplemental Figure S7B: Hierarchical Clustering of 10 arrays. All microarray data are based on six probe
replicates for each miRNA prediction (MCE-MIR, Cand and MIR) and eight probe replicates for mmu-mirs. ES and
GCNF samples represent RNA isolated from pooled material from two independent 10cm dishes with ~ 75 million
cells each from each time point of the ES and GCNF-/- time series experiment. For each time point, two dishes were
pooled to harvest 0.5-1.0 mg of RNA for the arrays. Each time point is represented by duplicate arrays with dye swap
of the label. The Adult Pool clearly separates as an independent group from ES and GCNF-/- ES Day 6 following
retinoic acid treatment (ESD6) and the GCNF-/- Day 6 following retinoic acid treatment (GCNFD6) appear to be more
related to each other as a separate group.  During Day 0, Day 1 and Day 3 the ES cells appear to be different from
GCNF-/- mutant during that period.