Slug is a direct Notch target required for initiation of cardiac cushion cellularization -- Data Supplement - JCB--Niessen et al. -- The Journal of Cell Biology

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Figure S1. Structural requirements for Notch induction of Slug expression. (A) Schematic of NotchICD deletion constructs used. A nuclear localization signal (NLS) was fused to ΔAnk and ΔRAM constructs to ensure proper localization of these mutants, as has previously been shown (MacKenzie, F., P. Duriez, B. Larrivee, L. Chang, I. Pollet, F. Wong, C. Yip, and A. Karsan. 2004. Blood, 104:1760–1768). (B) Immunoblotting for VE-cadherin, Slug, and Tubulin in HMEC overexpressing NotchICD deletion constructs.