Neuroglobin and Cytoglobin Distribution in the Anterior Eye Segment: A Comparative Immunohistochemical Study -- Ostojic et al. 56 (9): 863 Data Supplement - Supplemental Material - Ostojic et al. - Vol. 56, pp 863-872 -- Journal of Histochemistry and Cytochemistry

Neuroglobin and Cytoglobin Distribution in the Anterior Eye Segment: A Comparative Immunohistochemical Study
J. Histochem. Cytochem. Ostojic et al. 56: 863

Supplemental Material - Ostojic et al. - Vol. 56, pp 863-872

Online Supplemental data for Ostojic et al.: Supplemental Figures and Tables

Files in this Data Supplement:

Supplemental Table ST1 - Primary Antibodies Used in the Study.
Supplemental Table ST2 - Summary of the distribution of Ngb and Cygb in the human and canine anterior eye segment.
Supplemental Figure SF1 - Ngb and Cygb localization in human and canine iridal fibroblasts and melanocytes. Confocal images illustrating distribution patterns for (A) Ngb-IR (red) and (B) Vimentin-IR (green) in cells of the anterior border and stroma of the human iris. (C) Merged image illustrates colocalization of Ngb and vimentin within the same cells. (D) Corresponding brightfield image demonstrates pigmentation of some immunoreactive cells, as indicated with arrows. (E) Cygb-IR (red) and (F) Vimentin-IR (green) in cells of the anterior border and stroma of the human iris co-localized in (G). (H) Corresponding brightfield image showing pigmentation of some immunoreactive cells, as indicated with arrows. Confocal images illustrating (I) Ngb- IR (red) and (J) Vimentin-IR (green) in cells of the anterior border and stroma of the canine iris; immunoreactivity co-localized in (K). (L) Corresponding brightfield image; some immunoreactive cells are pigmented, as indicated with arrows. (M) Cygb- IR (red) and (N) Vimentin-IR (green) in cells of the anterior border and stroma of the canine iris are co-localized in (O). (P) Corresponding brightfield image showing that some immunoreactive cells are also pigmented, as indicated with arrows. In all images cell nuclei (blue) were labeled with DAPI. Scale bar 25 μm. Abbreviations: NE (nonpigmented epithelium), PE (pigmented epithelium), CM (ciliary muscle).
Supplemental Figure SF2 - Ngb and Cygb immunolocalization in the human ciliary muscle. Confocal images of the human ciliary muscle double-labeled for (A) Ngb (red) and (B) Cygb (green) shows immunoreactivity for both proteins in the ciliary muscle, trabecular meshwork and iris anterior border; immunostaining is co-localized, as illustrated in the merged image (C). In all images cell nuclei (blue) were labeled with DAPI. Scale bar 25 μm. Abbreviations: AC (anterior chamber), TM (trabecular meshwork), CM (ciliary muscle), IR (iris root).
Supplemental Figure SF3 - Ngb and Cygb immunolocalization in the human and canine lens. (A) Confocal images of the human lens double-labeled for (A) Ngb (red) and (B) Cygb (green) showed weak immunoreactivity in the lens epithelium, which is co-localized in the merged image (C). Confocal images illustrating (D) Ngb-IR (red) and (E) Cygb-IR (green) in canine lens co-localized in (F). In all images cell nuclei (blue) were labeled with DAPI. Scale bar 12.5 μm Abbreviations: LC (lens capsule), LE (lens epithelium).