Video 3
Triple imaging of GFP-DFCP1 (green), mRFP-MAP-LC3 (red), and CFP-ER (blue) during starvation showing formation of an autophagosomes from an omegasome (arrow). Clone 201 cells were transiently transfected with mRFP-MAP-LC3 and CFP-ER, then imaged in starvation medium at 1 frame per 10 s. The three panels are shown separately in addition to the triple overlay. Also see Fig. 6 A. The playback rate is at 10 frames per second.