Blood, Vol. 112, Issue 5, 1971-1980, September 1, 2008

Gossypol, a BH3 mimetic, induces apoptosis in chronic lymphocytic leukemia cells
Blood Balakrishnan et al. 112: 1971

Supplemental materials for: Balakrishnan et al

Files in this Data Supplement:

• Table S1. Patient information (PDF, 86.7 KB)
  
  
• Table S2. Effect of pan-caspase inhibitor on gossypol-induced apoptosis (PDF, 17.5 KB)
  
  
• Figure S1. Gossypol-induced PARP cleavage is not blocked by Z-VAD (JPG, 26.4 KB) -
  CLL cells were treated with or without Z-VAD in both the presence and absence of the indicated concentrations of gossypol for 24 hours. PARP protein was immunoblotted as described in Patients and Methods.
  
  
  
  
  
  
• Figure S2. Decrease in the ATP pool after treatment with gossypol (JPG, 59.5 KB) -
  CLL cells were treated with 30 µM gossypol for the indicated time. Nucleotides were extracted using the perchloric acid method and analyzed by high-pressure liquid chromatography as described in Patients and Methods.
  
  
  
  
  
  
• Figure S3. Gossypol treatment does not displace Mcl-1 and Bcl-2 into cytosol (JPG, 39.9 KB) -
  CLL cells were treated with or without gossypol at the indicated concentrations for 24 hours. Mcl-1 and Bcl-2 protein expression was studied in whole cell extract (WCE) and the cytosolic fraction using immunoblot method as described in Patients and Methods. Actin and GAPDH were used as loading controls.