EBNA1-specific T cells from patients with multiple sclerosis cross react with myelin antigens and co-produce IFN-{gamma} and IL-2 -- Data Supplement - JEM--Lunemann et al. -- The Journal of Experimental Medicine

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Figure S3. C. albicans antigen-specific, but not EBNA1-specific, CD4⁺ T cells produce IL-17. To investigate whether EBNA1-specific T cells produce IL-17, and whether the increased T cell response to EBNA1 in MS is mediated by Th17 rather than Th1 cells, CFSE-stained PBMCs from 6 patients with MS and 6 healthy donors were stimulated with pooled C. albicans antigens (40 µg/ml), or with EBNA1 or with SEB. After 7 d, cells were stimulated with PMA/ ionomycin for 24 h. Monensin was added for the last 12 h of incubation. The frequency of antigen-specific Th17 cells was determined by gating on the CD3⁺CD4⁺ CFSE^low population. 1–10% of C. albicans–specific T cells produced IL-17. IL-17 was not detectable in EBNA1-specific CD4+ T cells neither in MS patients nor in healthy blood donors.