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Figure S1. (A) CD4⁺ T cells from human peripheral blood were analyzed for expression of LAP and FoxP3. (B) AutoMACS-purified CD4⁺CD25⁺ (∼50% FoxP3⁺) and CD4⁺CD25⁻ T cells from human peripheral blood were activated with anti-CD3 and IL-2 and analyzed for FoxP3 and LAP expression at the indicated time points. One representative experiment out of three is shown.