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Figure S1. Deletion of Apc leads to lethality. (A) Efficiency of the targeted Apc deletion. Genomic DNA from primary Mx1-Cre⁺Apc^fl/fl, Mx1-Cre⁻Apc^fl/fl, Mx1-Cre⁺Apc^fl/+, and Mx1-Cre⁻Apc^fl/+ mice 4 d after three doses of pI-pC was analyzed by semi-qPCR to determine the proportion of Apc^fl and Apc^deleted alleles. (B) Kaplan-Meier survival curve of primary Mx1-Cre⁺Apc^fl/fl (n = 20), Mx1-Cre⁻Apc^fl/fl (n = 20), Mx1-Cre⁺Apc^fl/+ (n = 14), and Mx1-Cre⁻Apc^fl/+ (n = 14) mice. Arrowheads indicate pI-pC injections.