Moy et al. 10.1073/pnas.0711862105.
Fig. 7. Amino acid alignment of the N-terminal region of oyster bindin.
Fig. 8. C. gigas spermatogenic tissue expresses bindin mRNAs with between one and five F-lectin repeats. EcoRI-digested plasmid DNA from cloned PCR amplifications of the entire bindin ORF of a single individual is shown. Inserts differ in size by ~414 bp, the length of one F-lectin repeat.
Table 1
Fig. 9. Amino acid alignment of the full-length F-lectin repeats of oyster bindin.
Table 2
Table 3
Movie 1. Spatial locations of amino acid substitutions in oyster bindin repeats threaded on to the F-lectin fold crystal structure.
SI Text
Estimate of the Maximum Potential Number of Unique Bindin Molecules Generated by One Male by Alternate Splicing and Recombination
We assume that the individual has two genomic bindin alleles each with five repeats. Recombination in the intron of each repeat unit creates four possible combinations per repeat (two parental + two recombinant). Alternative splicing occurs between repeats, with the exception of the final repeat, whose connection to the 3'UTR is not spliced.
For mRNA molecules with a given number of repeats we get the expression:
MN = S(RN).
MN is the maximum number of unique mRNAs for a molecule with N repeats. S is the number of possible alternative splice combinations. R is the number of recombinants per repeat (two parental + two recombinant = four). N is the number of repeats.
One-Repeat Molecules
There are 5(41) = 20 possible combinations of one repeat mRNA molecules.
Parental Alleles
5'|--|--|--|f-|-r|3'
5'|--|--|f-|--|-r|3'
5'|--|f-|--|--|-r|3'
5'|f-|--|--|--|-r|3'
5'|--|--|--|--|FR|3'
5'|--|--|--|F-|-R|3'
5'|--|--|F-|--|-R|3'
5'|--|F-|--|--|-R|3'
5'|F-|--|--|--|-R|3'
Recombinant Alleles
5'|--|--|--|--|Fr|3'
5'|--|--|--|F-|-r|3'
5'|--|--|F-|--|-r|3'
5'|--|F-|--|--|-r|3'
5'|F-|--|--|--|-r|3'
5'|--|--|--|--|fR|3'
5'|--|--|--|f-|-R|3'
5'|--|--|f-|--|-R|3'
5'|--|f-|--|--|-R|3'
5'|f-|--|--|--|-R|3'
Two-Repeat Molecules
Only a single parental type is shown, M2 = 20(42) = 320.
5'|--|--|--|fr|fr|3'
5'|--|--|f-|-r|fr|3'
5'|--|f-|--|-r|fr|3'
5'|f-|--|--|-r|fr|3'
5'|--|--|fr|--|fr|3'
5'|--|f-|-r|--|fr|3'
5'|f-|--|-r|--|-r|3'
5'|--|fr|--|--|fr|3
5'|f-|-r|--|--|-r|3'
5'|fr|--|--|--|fr|3'
5'|--|--|fr|f-|-r|3'
5'|f-|--|-r|f-|-r|3'
5'|f-|-r|--|f-|-r|3'
5'|f-|-r|f-|--|-r|3'
5'|fr|--|f-|--|-r|3'
Three-Repeat Molecules
Only a single parental type is shown, M3 = 21(43) = 1,344.
5'|--|--|fr|fr|fr|3'
5'|--|f-|-r|fr|fr|3'
5'|--|fr|--|fr|fr|3'
5'|--|fr|f-|-r|fr|3'
5'|--|fr|fr|--|fr|3'
5'|--|fr|fr|f-|-r|3'
5'|f-|-r|fr|f-|-r|3'
5'|fr|--|fr|--|fr|3'
5'|fr|f-|-r|--|fr|3'
5'|fr|fr|--|--|fr|3'
5'|fr|f-|--|-r|fr|3'
5'|fr|--|f-|-r|fr|3'
5'|fr|--|--|fr|fr|3'
5'|f-|--|-r|fr|fr|3'
5'|f-|-r|--|fr|fr|3'
5'|f-|-r|f-|-r|fr|3'
5'|f-|-r|fr|--|fr|3'
5'|fr|--|fr|f-|-r|3'
5'|fr|f-|-r|f-|-r|3'
5'|fr|fr|--|f-|-r|3'
Four-Repeat Molecules
Only a single parental type is shown, M4 = 8(44) = 2,048.
5'|--|fr|fr|fr|fr|3'
5'|f-|-r|fr|fr|fr|3'
5'|fr|--|fr|fr|fr|3'
5'|fr|f-|-r|fr|fr|3'
5'|fr|fr|--|fr|fr|3'
5'|fr|fr|f-|-r|fr|3'
5'|fr|fr|fr|--|fr|3'
Five-Repeat Molecules
Only a single parental type is shown, M5 = 1(45) = 2,048.
5'|fr|fr|fr|fr|fr|3'
Total Possible Number of Potential Unique Bindin cDNAs
One repeat = 5(41) = 20
Two repeat = 20(42) = 320
Three repeat = 21(43) = 1,344
Four repeat = 8(44) = 2,048
Five repeat = 1(45) = 1,024
----------
Total = 4,756
Table 4