Resetting of FLOWERING LOCUS C expression after epigenetic repression by vernalization

Fig. 6. FLC-C24:GUS confers a late-flowering phenotype on the C24 ecotype. (A) The FLC-C24:GUS construct contains the FLC genomic construct (1) with the GUS coding region replacing the FLC stop codon. Exons are in black, and introns and 5' and 3' regions are in white. (B) This construct when introduced into C24 plants causes a modest delay in flowering. The delay in flowering is not as severe as an identical genomic FLC clone (lacking GUS gene) (1). Bars indicate the standard errors. (C) A 12-day-old nonvernalized plant (parent plant nonvernalized). (D) An 8-day-old plant vernalized for 56 days. (E) A 12-day-old nonvernalized plant (parent plant vernalized).

1. Finnegan EJ, Sheldon CC, Jardinaud F, Peacock WJ, Dennis ES (2004) Curr Biol 14: 911-916.

Table 2. Sequence of primers used