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Figure S5.  Equivalent frequencies of IL-10-producing CD4⁺ cells in CNV and GF mice. Cells isolated from the mLN (A), cecal patch (B) ,or large intestinal lamina propria (LPLs; C) were stimulated with PMA, ionomycin, and brefeldin A for 5 h ex vivo and stained for expression of CD4 and IL-10. (D) Expression of mRNA for Il10 in the large intestine of CNV and GF mice was analyzed by quantitative real-time PCR. Results are representative of 3 experiments (n = 12). Error bars indicate the SEM.