[View Larger Version of this Image]

Figure S6.  Equivalent expression of IL-12 and IFN-γ in CNV and GF mice. (A) mRNA expression of IL-12p35 (Il12a) in the large intestine of CNV and GF mice. Results are representative of 3 experiments (n = 12). (B) Cells isolated from mLN, cecal patch or large intestinal lamina propria (LPLs) were stimulated with PMA, ionomycin, and brefeldin A for 5 h ex vivo and stained for expression of CD4 and IFN-γ. Numbers represent frequency of IFN-γ⁺ CD4⁺ cells and are representative of 2 experiments (n = 8). Error bars indicate the SEM.