Transient high glucose causes persistent epigenetic changes and altered gene expression during subsequent normoglycemia -- Data Supplement - JEM--El-Osta et al. -- The Journal of Experimental Medicine

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Figure S1. Transient hyperglycemia causes a sustained increase in p65 gene expression caused by Set7-mediated histone methylation. (a) NF-κB p65 subunit mRNA levels after transient hyperglycemia in BAECs by quantitative RT-PCR. (b) Glucose dose–response curve for p65 expression in HAECs after 16-h exposure. (c) ChIP of NF-κB p65 promoter by antibodies to Set7 histone methyltransferase and to H3K4me1 in BAECs. *, P < 0.05 versus LG (5 mM); #, P < 0.01 versus LG. n = 4 for each graph. Error bars show SEM.