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Figure S2. Transient hyperglycemia causes a persistent increase in total p65 protein and in nuclear p65 activity. (a) HAECs were incubated with LG (5 mM glucose) or HG (30 mM glucose) for 16 h. After incubation in LG for the indicated times, total NF-κB p65 protein was measured by Western blotting and normalized against H3 as an internal control. *, P < 0.05 versus LG group. n = 4. Error bars are SEM. (b) After incubation in LG for the indicated times, cells were harvested for preparation of nuclear extracts, and the NF-κB p65 activity was measured using the Panomics Transcription Factor ELISA kit. *, P < 0.05 versus LG group. n = 3. Error bars show SEM.