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Figure S1. Conservation of SPDL-1 and other C. elegans genes in other organisms, and synthetic genetic interaction of SPDL-1 and SAC genes. (A) Gene names for C. elegans genes used in this study and their human homologues are shown. (B) An alignment of the amino acid sequences of a domain conserved among Spindly family proteins: SPDL-1 (NP_495637), C. briggsae hypothetical protein CBG19326B (XP_001674676), DmSpindly (Drosophila Spindly: NP_608791), and HsSpindly (human Spindly: NP_060255) is shown. (C) Effects of RNAi of the indicated gene on the viability of the strains carrying the mutant allele of SAC genes were analyzed. Hermaphrodites with indicated genotypes was fed HT115(DE3) bacteria carrying pUC19 (Ctrl) or those expressing double-stranded RNA (dsRNA) of indicated genes. Total numbers of eggs laid and viable progenies of three hermaphrodites were counted, and the percentages of viable progeny were calculated. For synthetic genetic analysis with mdf-1(gk2) allele, hermaphrodites hemizygous for mdf-1(gk2) allele, which was linked with unc-46(e177) and balanced over the translocation nT1(IV:V), [unc-46(e177) mdf-1(gk2)/nT1], were fed HT115(DE3) bacteria carrying pUC19 ((Ctrl) or those expressing dsRNA of indicated genes. Total numbers of wild-type–looking and Unc progenies (mdf-1(gk2) homozygotes) of 10 hermaphrodites were counted, and the percentages of Unc progenies were calculated. RNAi-mediated depletion of HCP-1, BUB-3, or GOA-1 were synthetically lethal with san-1Δ, mdf-1Δ, mdf-2Δ, and mdf-1(av19). san-1 and mdf-1, or san-1 and mdf-2, had a synthetic genetic interaction. spdl-1 has a synthetic genetic interaction with san-1 but not with mdf-1 or mdf-2. (D) Wild-type worms not injected (WT) or injected with dsRNA of spdl-1 were subjected to Western blotting with antibody to indicated proteins. Serial dilutions of wild-type extract were loaded to quantify the depletion level. More than 90% of endogeneous SPDL-1 was depleted.