Putting the model to the test: are APC proteins essential for neuronal polarity, axon outgrowth, and axon targeting? -- Data Supplement - JCB--Rusan et al. -- The Journal of Cell Biology

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Figure S3. APC mutant NBs exhibit normal polarity third instar larval brains. Antigens are indicated. (A and B) NBs fixed and stained for the apical marker aPKC and tubulin. (A) Wild-type NB. (B) APC2^g10 APC1^Q8 clone. (C and D) NB fixed and stained for the basal marker Miranda and tubulin. (C) Wild-type NB. (D) APC2^d40 APC1^Q8 clone. The insets are images of GFP::CD8, which positively marks the NB clones. (E–J) Clonal NBs are indicated by GFP staining (arrows), whereas adjacent wild-type NBs are indicated by arrowheads. (E–G) NBs fixed and stained for Armadillo (Arm) and actin. (H–J) NBs fixed and stained for Drosophila E-cadherin (DE-cad) and actin. (E and H) Control clones. (F and I) APC2^d40 APC1^Q8 clones. (G and J) APC2^g10 APC1^Q8 clones. Bars, 10 µm.