Blood, Vol. 112, Issue 8, 3138-3147, October 15, 2008

Unaltered repopulation properties of mouse hematopoietic stem cells transduced with lentiviral vectors
Blood Gonzalez-Murillo et al. 112: 3138

Supplemental materials for: Gonzalez-Murillo et al

Files in this Data Supplement:

• Table S1. Real-time quantitative PCR analysis of the exogenous hematopoietic reconstitution (cells positive for the SRY gene) in peripheral blood cells of mice B and F (PDF, 17.1 KB)
  
  
• Figure S1. Detailed analysis of expression in hematopoietic tissues of recipients transplanted with transduced chimeric grafts (JPG, 114 KB) -
  (A) Multilineage expression of the transgene (EGFP) in the PB of a representative mouse transplanted with chimeric transduced samples shown in Figure 1. Flow cytometry analyses of several differentiation antigens are shown: GR-1/MAC (myeloid lineage); B220 (B lymphoid lineage) and CD3 (T lymphoid lineage). Values correspond to the percentage of lineage differentiated cells (left quadrant) and the percentage of EGFP⁺ cells in the lineage differentiated cell population (right quadrant). (B) Percentage of Ly5.1⁺ cells and EGFP⁺ cells in different hematopoietic organs excised 9 months after primary transplants and 8 months after secondary transplants. Data obtained from primary and secondary recipients are shown. Each symbol corresponds to an individual mouse and the same symbol is used for primary and secondary recipients. Average values are presented as solid lines. PB: Peripheral blood, BM: Bone Marrow, Sp: Spleen and Thy: Thymus. (C) Analysis of the hematopoietic organs excised 9 months post-transplant of one recipient mouse. In this case, values correspond to the percentage of total Ly5.1⁺ cells (left quadrant) and the percentage of EGFP⁺ cells in the Ly5.1⁺ cell population (right quadrant).
  
  
  
  
  
  
• Figure S2. Correlation between provirus copy number per cell and percentage of EGFP⁺ cells in bone marrow samples from individual mice (n=28) (JPG, 56.5 KB) -
  Different symbols correspond to primary, secondary, tertiary or quaternary recipients as indicated in the legend.