Factors in Tissue Handling and Processing That Impact RNA Obtained From Formalin-fixed, Paraffin-embedded Tissue
J. Histochem. Cytochem. Chung et al. 56: 1033 Supplemental Material - Chung et al. - Vol. 56, pp 1033-1042Online Supplemental data for Chung et al.: Supplemental Table Files in this Data Supplement: - Supplemental Table ST1 - Oligonucleotide sequences of TaqMan probes and RT-PCR primers for targeting of GAPDH and HPRT
- Supplemental Figure SF1 - Standard curves of GAPDH (A) and CDK4 (B) by QuantiGene asaay. We performed Quantigene assay with mouse kidney FFPE-RNA ranging from 0.05 to 3 μg of total RNA. Data are from three independent experiments, and are expressed as mean ± SD. The assay showed great linearity (R2 > 0.99) according to amount of input RNA. Considering the dynamic range of standard curve, we applied 200 ng (for GAPDH) and 500 ng (for CDK4) of starting total RNA for the assay. Open and closed circles represent one-side and normal-side probes, respectively.
- Supplemental Figure SF2 - Histologic sections stained with H&E of the tissue locks after formalin fixation and paraffin embedding for the conditions of fixation time, fixative buffer and processor time examined. All images are total magnification 320X. Scale bar equal to 100 μm.
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