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Figure S5. The functional activity of neutralizing anti-TLR3 for inflammatory chemokine production is not dependent on endosomal acidification. (A and B) Chemokines were measured from peritoneal macrophages previously incubated without (−CL) or with (+CL) chloroquine and then exposed to poly(I:C) in the presence of anti-TLR3 or control IgG. Data are means ± SEM obtained from one experiment in triplicate (cells were pooled from five mice) and are representative of two independent experiments. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 compared with IgG-treated macrophages.