Video 3
Vps29-YFP on a tubule that emanates from a CFP-Rab7–positive endosome. HeLa cell cotransfected with plasmids encoding Vps29-YFP (green) and CFP-Rab7 (red) showing the localization of CFP-Rab7 on an endosome and the presence of only Vps29-YFP on a tubule that detaches from the same endosome. Merging green and red channels generated the third image on the right; yellow indicates overlapping localization of objects in the green and red channels. Time lapse is 121 s, with each frame taken every 1.2 s and displayed at 15 frames per second. Representative still images are shown in Fig. 3 C.