Video 4
Vps29-YFP and CFP-Rab5a localize to distinct endosomal domains. HeLa cell cotransfected with plasmids encoding Vps29-YFP (green) and CFP-Rab5a (red) showing the localization of each protein to different domains of endosomes. Merging green and red channels generated the third image on the right; yellow indicates overlapping localization of objects in the green and red channels. Time lapse is 155 s, with each frame taken every 1 s and displayed at 15 frames per second. Representative still images are shown in Fig. 3 D.