Supplementary material for Chang and Gould (2000) Proc. Natl. Acad. Sci. USA 97 (10), 5249-5254.
Table 1.
Strains used in this studyStrain designation | Genotype | Source |
Sz. pombe | ||
KGY137 | ade6-M210 /ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 h-/h+ | K. Gould |
KGY246 | ade6-M210 leu1-32 ura4-D18 h- | K. Gould |
KGY1234 | sid4 -SA1 ade6-M210 leu1-32 ura4-D18 h- | K. Gould |
KGY1340 | sid4::sid4-9xmyc[Kan R] ade6-M210 leu1-32 ura4-D18 h- | This study |
KGY1347 | leu1-32::pJK148 nmt1-sid4 DN ade6-M210 ura4-D18 h- | This study |
KGY1358 | sid4 +/sid4::ura4+ ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 h-/h+ | This study |
KGY1576 | sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h- | This study |
KGY1591 | sid1-239 sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h- | This study |
KGY1592 | sid2-250 sid4::sid4-3xHA[Kan R] ade6-M216 leu1-32 ura4-D18 h- | This study |
KGY1593 | spg1-106 sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h+ | This study |
KGY1594 | cdc7-24 sid4::sid4-3xHA[Kan R] ade6-M216 leu1-32 ura4-D18 h+ | This study |
KGY1595 | cdc11-123 sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h- | This study |
KGY1596 | cdc14-118 sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h+ | This study |
KGY1597 | cdc15-140 sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h+ | This study |
KGY1598 | cdc16-116 sid4::sid4-3xHA[Kan R] ade6-M216 leu1-32 ura4-D18 h+ | This study |
KGY1599 | nda3-km311 sid4::sid4-3xHA[Kan R] ade6-M210 leu1-32 ura4-D18 h- | This study |
KGY1600 | cdc7::cdc7-3xHA[ura4 +] sid4-SA1 ade6-M216 leu1-32 ura4-D18 h- | This study |
KGY1725 | spg1::spg1-3xHA[ura4 +] sid4-SA1 ade6-M210 leu1-32 ura4-D18 h- | This study |
KGY1741 | spg1::spg1-3xHA[ura4 +] leu1-32::pJK148 nmt1-sid4DN ade6-M210 ura4-D18 h- | This study |
KGY1742 | sid4::sid4-3xHA[Kan R] leu1-32::pJK148 nmt-sid4DN ade6-M210 ura4-D18 h- | This study |
KGY2171 | byr4::ura4+ leu1-32:: pJK148 nmt41-byr4+ ura4-D18 leu1-32 h- | C. Albright |
KGY2732 | sid4-myc /sid4-HA KGY137 | This study |
Sc. cerevisiae | ||
KGY1400 | YPB2: MATa ura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu2-3,112 canR gal4-542 gal80-538 LYS2::GAL1uas-LEU2TATA-HIS3 URA3::(GAL417mersx3)-CYC1TATA-lacZ | S. Hemmingsen |