Blood -- Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5

Blood, Vol. 112, Issue 13, 4905-4914, December 15, 2008

Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5
Blood Schulteis et al. 112: 4905

Supplemental materials for: Schulteis et al

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Figure S1. Gimap5^−/− mice lack DX5^POSNK1.1^POS NK cells (JPG, 91.9 KB) -
Single cell suspensions were prepared from the indicated tissues of wild type (WT; upper panel) and gimap5^−/− mice (−/−; lower panel) and stained with anti–NK1.1-Cy7 and anti–DX5-FITC antibodies. NK1.1 versus DX5 plots of fluorescence intensity represent cells comprised by the lymphocyte gate imposed on forward/side scatterplots. Representative examples from one wild type and one gimap5^−/− mouse are shown.
Figure S2. Emergence of gimap5^−/− NK and NK–T-cell populations in sublethally irradiated Jak3^−/− recipients (JPG, 74.6 KB) -
NK-deficient Jak3^−/− mice were sublethally irradiated and transplanted with unfractionated bone marrow from wild type (wt) or gimap5^−/− (−/−) mice. Gimap5^−/− marrow gives rise to CD3^POSCD49b^POS NK-T and CD3^NEGCD49b^POS NK cells.